Dysregulations of 26 microRNAs and 14 genes were used to propose novel biomarkers of LEAD. Discriminative value of revealed microRNAs and genes were confirmed by receiver operating characteristic analysis. Statistical significance of microRNAs and genes analysis results was evaluated using DESeq2 and uninformative variable elimination by partial least squares methods. High-throughput sequencing was employed to examine microRNAome and transcriptome of peripheral blood mononuclear cells of patients with LEAD, in relation to controls. We aimed to indicate the most promising microRNA and gene expression signatures of LEAD, to identify interactions between microRNA and genes and to describe potential effect of modulated gene expression. MicroRNAs belong to epigenetic factors that regulate gene expression and have not yet been extensively studied in LEAD. Lower extremities arterial disease (LEAD) is a common manifestation of atherosclerotic disease of arteries of lower extremities. 6Department of Vascular Surgery and Angiology, Medical University of Lublin, Lublin, PolandĪtherosclerosis and its comorbidities are the major contributors to the global burden of death worldwide.5Chair and Department of Medicinal Chemistry, Medical University of Lublin, Lublin, Poland.4Department of Pathology and Laboratory Medicine, Rutgers–Robert Wood Johnson Medical School, New Brunswick, NJ, United States.3Ecotech Complex, Analytical and Programme Centre for Advanced Environmentally-Friendly Technologies, University of Marie Curie-Sklodowska, Lublin, Poland.2Department of Clinical Genetics, Chair of Medical Genetics, Medical University of Lublin, Lublin, Poland.1Chair and Department of Biology and Genetics, Medical University of Lublin, Lublin, Poland.Ruszel 2†, Andrzej Stępniewski 3, Dariusz Gałkowski 4, Jacek Bogucki 2, Łukasz Komsta 5, Przemysław Kołodziej 1, Tomasz Zubilewicz 6, Marcin Feldo 6‡ and Janusz Kocki 2‡
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